Nucleic Acids Research, 2008, Vol. 36, No. Supplement 2, pp W163-W169.
IDT SciTools: a suite for analysis and design of nucleic acid oligomers
Richard Owczarzy, Andrey V. Tataurov, Yihe Wu, Jeffrey A. Manthey,
Kyle A. McQuisten, Hakeem G. Almabrazi, Kent F. Pedersen, Yuan Lin,
Justin Garretson, Neil O. McEntaggart, Chris A. Sailor, Robert B. Dawson,
and Andrew S. Peek
Free reprint
DNA and RNA oligomers are used in a myriad of diverse biological and
biochemical experiments. These oligonucleotides are designed to have unique
biophysical, chemical and hybridization properties. We have created an
integrated set of bioinformatics tools that predict the properties of native
and chemically modified nucleic acids and assist in their design. Researchers
can select PCR primers, probes and antisense oligonucleotides, find the most
suitable sequences for RNA interference, calculate stable secondary structures,
and evaluate the potential for two sequences to interact. The latest, most
accurate thermodynamic algorithms and models are implemented. This free
software is available at http://www.idtdna.com/SciTools/SciTools.aspx.
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