Proc. Natl. Acad. Sci. USA, 2003, Vol. 100, No. 25, pp 14840-14845.
Thermodynamic treatment of oligonucleotide duplex-simplex equilibria
Richard Owczarzy, Isard Dunietz, Mark A. Behlke, Irving M. Klotz and Joseph A. Walder
Free reprint
Thermodynamic formulations have been devised to obtain ΔGo values directly
from spectroscopic data at a fixed common temperature in nucleic acid
duplex-simplex melting curves. In addition, the dependence of melting on
salt concentration has been expressed in terms of a stepwise stoichiometric
representation, which leads to a specific equation for the partition of
the added sodium ions between the different oligonucleotide forms.
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